User:Steven J. Koch/Notebook/Kochlab/2008/07/16/Lambda DNA visualization

• Aliquoted YOYO1 into 20 ul aliquots ... now stored at -20C

• Making 10x YOYO1 stain:
  • 999 ul TE buffer (from Sandia)
  • 1 ul YOYO1 stock
  • covered tube partially with masking tape, is in drawer

• Made working DNA solution
  • 88 ul TE buffer
  • 1 ul Taq
  • 1 ul lambda DNA stock (vortexed and heated by hand...was still very viscous)
  • 10 ul 10x YOYO solution (above)
  • Did not see any stretched out DNA, but PLENTY of DNA balled up in solution. Fading bad, but not too bad.

• Adding 10 ul taq to the remaining about 75 ul of the above working solution.
  • The sample leaked (or probably I flowed it around the edge), so we made a new sample, and watched as we flowed in the new higher taq
  • Did not look any different than with less taq. Not proving anything, but best guess is that taq is not working like the RT was.