User:Spenser Skates/Notebook/pTKX luciferase biobrick/2008/05/02
|Project name|| Main project page|
Previous entry Next entry
Incorporation of PCR BB product into backbone (2nd attempt)
Cut PCR results and three different backbones (amp+kan, amp+cm, amp+tet) seperately with EcoRI and PstI, then denatured restriction enzymes. Ligated DNA together with T4 DNA ligase.