User:Spenser Skates/Notebook/pTKX luciferase biobrick/2008/04/28

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

Incorporation of PCR BB product into backbone

Cut PCR results and Kan backbone seperately with EcoRI and PstI, then denatured restriction enzymes. Ligated DNA together with T4 DNA ligase.
Knight:DNA_ligation_using_T4_DNA_ligase
Transformed ligated product into E.coli by heatshock
Transforming_chemically_competent_cells_(Inoue)
Plated transformants on amp+kan plate.


Personal tools