User:Spenser Skates/Notebook/pTKX luciferase biobrick/2008/04/23

From OpenWetWare
Jump to navigationJump to search
Project name Main project page
Previous entry      Next entry

Turned operon into biobrick part with PCR

Used PCR to turn the sequence containing the ORF's lux A-E into a biobrick part. Used the following primers:

GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGGAGgcaaatatgactaaaaaaatttcattcattattaacg,luxF
GTTTCTTCTACTAGTAGCGGCCGCTGCAGtcaactatcaaacgcttcggttaag,luxR