User:Shahar Sukenik/Notebook/p53CT expression in minimal media/2010/08/02

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02/08/10: Starter

1. Get colony from petri dish, put in 2-3 ml in LB + amp (1:1000 amp) 2. Grow O/N on shaker, 220 rpm 37C.


Preps for day 3:

1. 2 500 ml Erlenmeyer to prepare M9 media

2. 5 250 ml Erlenmeyer for growing

3. Stocks to prepare mediums:

  • 1. 5xM9 salts
  • 2. 2xYT + NPS
  • 3. 20% glucose
  • 4. 1M MgSO4
  • 5. 1M CaCl2
  • 6. 200 ml DDW


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