User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/07/11

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Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.

  • Performed transformation of yesterday's ligation products into JM109 competent cells to obtain:
    1. pBEST-PB-UTR1-deGFP-T500
    2. pBEST-PD-UTR1-deGFP-T500
    3. pBEST-PF-UTR1-deGFP-T500
    4. pBEST-PG-UTR1-deGFP-T500
    5. pBEST-PL-UTR1-deGFP-T500
    6. pBEST-PL-L-PA-UTR1-deGFP-T500
    7. -linker control

Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.

  • Performed transformation of yesterday's ligation products into JM109 competent cells to obtain:
    1. pBEST-PB-UTRB-deGFP-T500
    2. pBEST-PD-UTRD-deGFP-T500
    3. pBEST-PF-UTRF-deGFP-T500
    4. pBEST-PG-UTRG-deGFP-T500
    5. pBEST-PL-UTRL-deGFP-T500
    6. -linker control

Hypothesis 2: Gene L is necessary for phage propagation.

  • On hold until new primers arrive.



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