User:Saroj Pandey/Notebook/SNP PCR optimization/2014/09/25
|Project name|| Main project page|
Previous entry Next entry
Allele Specific PCR and Multiplex PCR
• No bands are seen at 992bp which tells that amplification with the outer primers was not successful
• Faint bands are seen at about 500bp in both the taster and the non-taster
• As observed in previous PCRs, a thick bright band is seen below 100bp. But there is also another band at approximately 150bp.
• Since the expected products are of similar lengths, this PCR is unable to distinguish between the two variants and other set of primers could be used