User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/10/04

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

UV Vis Silver Round 1

pH 4 - 12, Fructose 0 mM, 0.0625 mM

Solutions

  • prepared solutions with HCl, which made our samples ph 4 - 6 useless (AgCl forms and precipitates)

we discarded pH 4 - 6 solutions and only measured absorbance for pH 7 - 12. Observations: all except pH 12 show precipitates. only pH 12 is a homogeneous, slightly orange solution.


volumes and concentrations used:

Fructose = 0.0 mM

Image:Screenshot 2016-10-04 at 1.14.47 PM.png

Fructose = 0.0625 mM

Image:Screenshot 2016-10-04 at 1.17.42 PM.png

we prepared pH 4 - 6 again, with HNO3

Image:Screenshot 2016-10-04 at 1.20.38 PM.png

Image:Screenshot 2016-10-04 at 1.21.26 PM.png


The above screen - shotted spreadsheet can be found in the Dropbox under "AG Stock Solution Calculation Template"

We finished the UV Vis on Wednesday.

Results

Image:0Fructose October 4th A530.PNG

The above chart shows the UV-Vis spectra for [Fructose]=0 mM, [Ag]=0.25 mM. Nanoparticles were only formed for pH 4, 11, and 12. All other pH solutions formed a cream colored precipitate, and did not appear to form nanoparticles from the spectra.


Image:0.0625Fructose October4thspectrum.PNG

The above chart shows the UV-Vis spectra for [Fructose]=0.0625 mM, [Ag]=0.25 mM. As with 0 mM fructose, nanoparticles were only formed for pH 4, 11, and 12. All other pH solutions formed a cream colored precipitate, and did not appear to form nanoparticles from the spectra.


Image:A530 0 fructose redo redo ok.PNG

The absorbance at 530 nm is shown above for [Fructose]=0 mM, [Ag]=0.25 mM. As expected, solutions that did not form nanoparticles have an absorbance of ~0.


Image:0.0625Fructose October4thA530.PNG

The absorbance at 530 nm is shown above for [Fructose]=0.0625 mM, [Ag]=0.25 mM. As expected, solutions that did not form nanoparticles have an absorbance of ~0.




Personal tools