User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/09/27

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Ocean Optics Round 3

pH 6

BSA Solution

  1. BSA Ocean Optics Solution
    1. pH 6
    2. [Au]=0.25 mM
    3. [BSA]=3.125 uM
    4. Vf=3000 uL
  2. Stock Concentrations 160927
    1. [BSA]= 52.6 uM
    2. [Au]= 4.27 mM
    3. [HCl]= 1mM
  3. Volumes for making the solution:
    1. 3 uL HCl
    2. 294.6 uL BSA
    3. 292.7 uL Au
    4. 2409.5 uL HOH

Results

Image:OO pH6 part1 160928.PNG

The chart above shows a dramatic increase in maximum wavelength from about ~450 to ~550 at about ~3200s.

Image:OO pH6 part3 160928.PNG

The chart above shows a slight decrease in max absorbance until about 1200 sec. at which point it dramatically increases for the rest of the run.

Image:OO pH6 part2 160928.PNG

The chart above shows a slight decrease in A(535) until about 1200 sec. at which point it dramatically increases for the rest of the run. This is similar to the maximum absorbance over time chart as expected.



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