User:Sarah A Boswell
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Sarah A Boswell
Harvard Medical School
200 Longwood Ave.
Boston, MA 02115
- 2003, PhD, Rensselaer Polytechnic Institute
Dissertation: The folding of the dimeric protein factor for inversion stimulation
- 1999, BS, Rensselaer Polytechnic Institute
Research Project in the Springer Lab - FLEXIQuant
We have developed a novel mass spectrometry method that promises to be sufficiently cheap, straightforward and reliable to provide high-throughput measurements of the absolute level of proteins in cells and tissues, and at the same time both identify peptides that are post-translationally modified and quantite the level of modification. Our primary goal in this proposal is to comprehensively characterize 1,000 proteins and thereby to demonstrate that it is possible to scale the technology to make such measurements at a quasi-genomic level across multiple cell types (6 cell lines).
Other Research interests
- Protein folding and misfolding
- The cellular response to reactive oxygen stress (ROS)
- The unfolded protein response (UPR)
1: Muñiz VA, Srinivasan S,Boswell SA, Meinhold DW, Childs T, Osuna R, Colón W. The role of the local environment of engineered Tyr to Trp substitutions for probing the denaturation mechanism of FIS. Protein Sci. 2011 Feb;20(2):302-12.
2: Hwang SO, Boswell SA, Seo JS, Lee SW. Novel oxidative stress-responsive gene ERS25 functions as a regulator of the heat-shock and cell death response. J Biol Chem. 2008 May 9;283(19):13063-9.
3: Das S, Boswell SA, Aaronson SA, Lee SW. P53 promoter selection: choosing between life and death. Cell Cycle. 2008 Jan 15;7(2):154-7. Review.
4: Boswell SA, Ongusaha PP, Nghiem P, Lee SW. The protective role of a small GTPase RhoE against UVB-induced DNA damage in keratinocytes. J Biol Chem. 2007 Feb 16;282(7):4850-8.
5: Ongusaha PP, Kim HG, Boswell SA, Ridley AJ, Der CJ, Dotto GP, Kim YB, Aaronson SA, Lee SW. RhoE is a pro-survival p53 target gene that inhibits ROCK I-mediated apoptosis in response to genotoxic stress. Curr Biol. 2006 Dec 19;16(24):2466-72.
6: Lynch SM, Boswell SA, Colón W. Kinetic stability of Cu/Zn superoxide dismutase is dependent on its metal ligands: implications for ALS. Biochemistry. 2004 Dec 28;43(51):16525-31.
7: Chen CH, Battaglioli G, Martin DL, *Hobart SA, Colón W. Distinctive interactions in the holoenzyme formation for two isoforms of glutamate decarboxylase. Biochim Biophys Acta. 2003 Jan 31;1645(1):63-71.
8: *Hobart SA, Meinhold DW, Osuna R, Colón W. From two-state to three-state: the effect of the P61A mutation on the dynamics and stability of the factor for inversion stimulation results in an altered equilibrium denaturation mechanism. Biochemistry. 2002 Nov 19;41(46):13744-54.
9: *Hobart SA, Ilin S, Moriarty DF, Osuna R, Colón W. Equilibrium denaturation studies of the Escherichia coli factor for inversion stimulation: implications for in vivo function. Protein Sci. 2002 Jul;11(7):1671-80.
- Writing under maiden name