User:Sara K. Mattson/Notebook/Chemistry 671/2016/10/19

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UV-VIS Purpose To observe the change in absorbance of AgNO3 NP's at pH 4-12 for fructose at concentrations 0.125mM and 0.25mM Procedure Solutions were prepared on 10/18/2016 The solutions had been heated in an 80C oven for four hours and then sat at room temperature. UV-Vis spectra were taken of each sample from 200-800nm. The test tubes were kept completely wrapped in tin foil the entire time, because AgNO3 is light sensitive. Solutions All of the solutions for both concentrations of fructose were cloudy. Below are photos of pH 11 and 12 respectively, going in order from 0mM Fructose- 0.25mM fructose Ag NP's, myoglobin, pH11: 0mM, .0625mM, .125mM, .25mM Fructose Ag NP's, myoglobin, pH12: 0mM, .0625mM, .125mM, .25mM Fructose Data Graphs for .125mM Fructose Absorbance v. Wavelength Absorbance at 272nm Absorbance at 400nm Graphs for 0.25mM Fructose Absorbance v. Wavelength Absorbance at 396nm Absorbance at 284nm