User:Sara K. Mattson/Notebook/Chemistry 671/2016/09/28

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UV-VIS

Purpose

To observe the change in absorbance at pH 4-12 for fructose at concentrations 0.25mM, 0.75mM and 1.25mM

Procedure

The solutions were made 9/27/2016

The solutions had been in 80C oven for four hours and then sat at room temperature. UV-Vis spectra were taken of each sample from 200-800nm.


0.25mM Fructose pH12-4 (left to right)

0.75mM Fructose pH 12-4 (left to right)

1.25mM Fructose pH 4-12 (left to right)

Results

  1. Graphs for 0.25mM Fructose

Absorbance v. Wavelength

Absorbance at 529nm

Absorbance at 394nm

  1. Graphs for 0.75mM Fructose

Absorbance v. Wavelength

Absorbance at 529nm

Absorbance at 392nm

  1. Graphs for 1.25mM Fructose

Absorbance v Wavelength

Absorbance at 529nm

Absorbance at 410nm



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