User:Robwarden/Notebook/Divalent Ligand Validation/2009/03/20

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pMSCVN-H1C Validation

Expected Banding Patterns

Correct
Size site1 site2 Mass %
6587 EcoRI 5834 EcoRI 1720 62
1838 EcoRI 1720 EcoRI 3558 17
1508 EcoRI 4326 EcoRI 5834 14
768 EcoRI 3558 EcoRI 4326 7
Image:PMSCVN-H1C-EcoRI.png
Empty
Size site1 site2 Mass %
6303 EcoRI 1436 EcoRI 1436 100
Image:PMSCVN-EcoRI.png

Miniprep

EcoRI Digest

 10 μL DNA
  2 μL 10X Buffer EcoRI -> 26 μL
  1 μL EcoRI            -> 13 μL
  7 μL MilliQ           -> 91 μL

Incubate at 37°C for 1hr.

Results

  • No correct colonies
  • Will redo cloning strategy next week
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