User:Robwarden/Notebook/Caspase-3 Activatable Probe/2009/09/14

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Colony Screen

Miniprep

with Qiagen Kit

Digestion

  • EcoRI & SgrAI (.5 μL each)
  • 10 μL colony DNA (or 1 μg control plasmid)
  • NEB Buffer 4

Gel Electrophoresis

Top Wells
Lane 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Sample 5x Control 1kb R-5-1 R-5-2 R-5-3 R-5-4 R-5-5 R-5-6 100 bp G-5-1 G-5-2 G-5-3 G-5-4 G-5-5 G-5-6
Bottom Wells
Lane 1 2 3 4 5 6 7 8
Sample 1x Control 1kb R-1-1 R-1-2 R-1-3 R-1-4 R-1-5 R-1-6
  • Naming Scheme:
    • G = hgLuc, R = hRLuc8
    • 1 = 1xDEVD, 5 = 5xDEVD
    • Final Number = Colony Number

Results

It didn't look like anything cut, so I probably messed up the restriction digest... I sent the first two colonies in for sequencing anyway, since the previous screen gave high yield.


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