User:Robwarden/Notebook/Caspase-3 Activatable Probe/2009/09/14

Caspase-3 Activatable Probe

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Colony Screen Miniprep with Qiagen Kit Digestion EcoRI & SgrAI (.5 μL each) 10 μL colony DNA (or 1 μg control plasmid) NEB Buffer 4 Gel Electrophoresis Top Wells Lane 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Sample 5x Control 1kb R-5-1 R-5-2 R-5-3 R-5-4 R-5-5 R-5-6 100 bp G-5-1 G-5-2 G-5-3 G-5-4 G-5-5 G-5-6 Bottom Wells Lane 1 2 3 4 5 6 7 8 Sample 1x Control 1kb R-1-1 R-1-2 R-1-3 R-1-4 R-1-5 R-1-6 Naming Scheme: G = hgLuc, R = hRLuc8 1 = 1xDEVD, 5 = 5xDEVD Final Number = Colony Number Results It didn't look like anything cut, so I probably messed up the restriction digest... I sent the first two colonies in for sequencing anyway, since the previous screen gave high yield.