User:Roberta Diaz Jimenez/Notebook/CHEM 472/2016/01/27

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Objective

The objective for this experiment is to prepare gold and silver nanoparticles using soy protein isolate and casein

Materials

SPI (Dr. Fox's Lab)

Casein (Dr. Fox's Lab)

Procedure

Gold and Silver NPs Procedure to make the gold nanoparticles is adapted Dr. Hartings' notebook. The molarity of protein was used as well as gold. Due to SPI not having a clear average molecular weight, molarity of Casein is recorded in mg/ml and same concentration used for SPI.

Reaction Setup Detailed Below

' 2.5mM HAuCl4 2.5mMAgNO3 .44mg/ml SPI .44mg/ml Casein 2.5mM Fructose Water Au/Ag:Protein Notes
140µL00560µL0400µL1:15no color change
240µL00440µL0520µL1:11
340µL00360µL0600µL1:09
440µL00280µL0680µL1:07
540µL00200µL0760µL1:05
6040µL0560µL0uL400µL1:15all gold/bright yellow except for #14 which was lighter
7040µL0560µL20uL380µL1:15
8040µL0560µL40uL360µL1:15
9040µL0360uL0uL600µL1:09
10040µL0360µL20uL580µL1:09
11040µL0360µL40uL560µL1:09
12040µL0200uL0uL760µL1:05
13040µL0200µL20uL740µL1:05
14040µL0200µL40uL720µL1:05
1540µL0560µL00400µL1:15Made fibers so need higher concentration of protein
1640µL0440µL00520µL1:11purple fibers at bottom
1740µL0360µL00600µL1:09
1840µL0280µL00680µL1:07
1940µL0200µL00760µL1:05
20040µL560µL00uL400µL1:15No color change
21040µL560uL020uL380µL1:15
22040µL560µL040uL360µL1:15
23040µL360uL00uL600µL1:09
24040µL360µL020uL580µL1:09
25040µL360µL040uL560µL1:09
26040µL200uL00uL760µL1:05
27040µL200µL020uL740µL1:05
28040µL200µL040uL720µL1:05
2940µL0000960µLNo color change
30040µL000960µL

Results

  1. All casein samples changed from clear to a bright golden yellow, indicating NP formation. This occurred regardless of protein volume added or fructose content.
  2. SPI gold samples formed purple dots at bottom of tube, indicating some nanoparticle or nanofiber formation. Possible precipitation of protein as well.
  3. SPI silver and casein gold samples remained clear
  4. Detailed results can be found: SRB Lab Notebook Data for this entry's date


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