User:Roberta Diaz Jimenez/Notebook/CHEM 471 Experimental Lab/2015/11/11

Project name

Procedure

Centrifuged falcon tubes containing gold fibers.
Removed water with pipette.
Prepare the sample that goes in the 3mL cuvette that will be used for ocean optics.
The sample must contain:
1. Gold fibers
2. (2.996mL) of Tris buffer
3. (0.003994mL) of trypsin, calculations are below
4. The trypsin was added last, at the second reading such that second reading becomes time 0.
We ran our sample overnight.
Readings were taken every 20 minutes overnight. The data was uploaded directly to DropBox for later analysis.

Calculations for volume of trypsin, and volume of buffer

    C1·V1 = C2·V2,
     where (C1 = 50.21µM)
           C2 = 1µM 
           V2 = 1000µL
    (V1 = 0.0199mL)

    C1·V1 = C2·V2,
     where (C1 = 1µM)
           C2 = 0.01µM 
           V2 = 3000µL
    (V1 = 30µL)