User:Roberta Diaz Jimenez/Notebook/CHEM 471 Experimental Lab/2015/10/13
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Measure protease Trypsin's kinetics with the fluorescence assay using 1µM of trypsin at different heating intervals.
The general protocol detailed in Dr. Hartings' notebook was used. The following specific steps were performed:
V1 = [(1µM)(1mL)]/77.68µM = 0.01287mL, amount of trypsin solution needed Volume of buffer: 1mL - 0.01287mL = 0.98713mL