User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/02/23

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Spermatogenesis

  • Dissected and visualized heat shocked flp-out flies for confirmation
    • Fix 20' in formaldehyde solution.
    • Wash 3 x 5'
    • Incubate in 0.6% Triton w/ PBS
    • Wash 1x with PBS-T
    • Mount
  • Set up cross to re-introduce balancer for control cross
    • FM7/Y; Sc/Cyo, 42D/CyoAg X HsFLP, MH107; mRFP, myb+, 42D/CyoAG
    • Will collect non Sc, Bar-eyed males and females.


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