User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/01/25

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Drosophila melanogaster Genetics Main project page
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Spermatogenesis

  • Mount ant-sa and anti-bam samples
  • Stain and mount totpro sample
    • Stain with ToPro3 1:1000 in 2% NGS
      • 30 mins at RT
    • Wash 4 x 5 minutes in PBST
    • Mount in Vectashield
  • PCR of recombinants(?) with flpase primers
    • Run normal PCR with 94C melting phase, 52C annealing phase, and 45' 72C extension phase
    • 2x normal magnesium
    • 4 reactions: -, +, exp1, and exp 9.
    • Expecting 700bp band


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