User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/01/20

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Spermatogenesis

  • Collected tdt-Myb virgin females
  • Set up gfp-PCNA on 3 x tdt-Myb virgins.

  • Collected and began dissection/staining protocol with gfp-Myb, rfp-H2AV flies
    • Will stain with anti-bam, anti-sa, and tdt-myb
  • Dissect testes in 1x PBS Solution. Store in eppendorf with 1x PBS until dissections are finished.
  • Fix with 4% Formaldehyde PBS Solution
    • 20 minutes
  • Wash 3 x 5 minutes in 1x PBS-T
  • Incubate 30 minutes with PBS 0.6% Triton.
  • Wash once with 1x PBS-T
  • Block in 2% NGS
    • O/N at 4C


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