User:Raf Aerts/Notebook/Open Coffee/2009/02/12

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Coffea arabica DNAx: low concentration, low purity

  • Nanodrop ND-1000 Spectrophotometer setup
    • nucleic acid DNA-50
    • blank: 1.5 μL CE buffer
    • samples 1.5 μL
  • Results
    • All 260/230 and all but one 260/280 ratios below 1.8 (low purity)
    • Most probably poor sample quality (leaves molded, black) and samples were also unsufficiently ground.

Image:Nanodropgraph.jpg

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