Parts assembly/ PCRs
ABSTRACT
- PCRs of HydEF1 and HydEF2.
- Today I did 8 PCR reactions, 4 to amplify HydEF1 and another 4 to amplify HydEF2, using the following proportions:
H2O
|
23μL
|
Phusion GC Buffer
|
10 μL
|
dATP
|
1 μL
|
dGTP
|
1 μL
|
dCTP
|
1 μL
|
dTTP
|
1 μL
|
Primer fwd
|
5 μL
|
Primer rev
|
5 μL
|
DNA template
|
1 μL
|
DMSO
|
1.5 μL
|
Phusion HF pol
|
0.5 μL
|
Cycles
|
Gel Lanes
|
Gel
|
- 95°C 5min
- 98°C 10s 30Cy
- 60°C 30S 30Cy
- 72°C 2 min 30Cy
- 72°C 10min
- 4°C
|
- Lane1 Ladder
- Lane2 HydEF1
- Lane3 HydEF1
- Lane4 HydEF2
- Lane5 HydEF2
|
|
Cycles
|
Gel Lanes
|
Gel
|
- 95°C 5min
- 98°C 10s 30Cy
- 62°C 30S 30Cy
- 72°C 2 min 30Cy
- 72°C 10min
- 4°C
|
- Lane1 Ladder
- Lane2 HydEF1
- Lane3 HydEF1
- Lane4 HydEF2
- Lane5 HydEF2
|
|
|