User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/09/21

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Parts assembly/ PCRs

ABSTRACT

  • PCRs of HydEF1 and HydEF2.

  • Today I did 8 PCR reactions, 4 to amplify HydEF1 and another 4 to amplify HydEF2, using the following proportions:
H2O 23μL
Phusion GC Buffer 10 μL
dATP 1 μL
dGTP 1 μL
dCTP 1 μL
dTTP 1 μL
Primer fwd 5 μL
Primer rev 5 μL
DNA template 1 μL
DMSO 1.5 μL
Phusion HF pol 0.5 μL
Cycles Gel Lanes Gel
  • 95°C 5min
  • 98°C 10s 30Cy
  • 60°C 30S 30Cy
  • 72°C 2 min 30Cy
  • 72°C 10min
  • 4°C
  • Lane1 Ladder
  • Lane2 HydEF1
  • Lane3 HydEF1
  • Lane4 HydEF2
  • Lane5 HydEF2


Cycles Gel Lanes Gel
  • 95°C 5min
  • 98°C 10s 30Cy
  • 62°C 30S 30Cy
  • 72°C 2 min 30Cy
  • 72°C 10min
  • 4°C
  • Lane1 Ladder
  • Lane2 HydEF1
  • Lane3 HydEF1
  • Lane4 HydEF2
  • Lane5 HydEF2





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