Gel of Helena´s PCR
- Last week Helena made a PCR using a Taq polymerase. She also used different concentrations of plasmid in order to see if the problem with the previous PCRs (the ones that I did) was the plasmid concentration. Today I run an electrophoresis gel at 120V for 40 min with the five samples she did, the results were not good because the only thing you could see in the gel was the DNA ladder. So our next approach is to isolate the fragment we need, of a band of a gel. First we have to digest the plasmid with the propper enzymes and then run an electrophoresis gel an isolate the fragment.