User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/07/18
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To make a PCR with rTth enzyme you have to prepare the following reactions:
The next step it tu put Mix 1 to warm at 94°C for 5 min, one second before the first cycle starts, stop the thermocycler and add to the first tube the 20 μL of Mix 2. This is called a Hot start. I left the PCR 35 cycles.