User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/06/02
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Because the E. coli strain used doesn´t have high nuclease content we did not perform the optional wash step.
After obtaining the samples, we did an electrophoresis with a 1% agarose gel, 80 V x 100 min. The proportions of the samples were 3μL of dye and 5 μL of plasmid DNA. Unfortunately the gel showed no visible bands in the lanes of plasmid DNA.
We did another electrophoresis using again a 1% agarose gel, 125 V X 30 min, with other sample proportions: in one lane 3μL of dye and 7μL of plasmid DNA, in a second lane 3μL of dye and 10μL of plasmid DNA. This new gel showed two bands, but not very clear that correspond to chromosome DNA. You can look for the picture in Pablo´s notebook.
The conclusion of this experiments is that something wrong is going on with the plasmid extraction kit. On monday we will try to find out what is wrong.