User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/11

From OpenWetWare
Jump to navigationJump to search
Project name Main project page
Previous entry      Next entry

Synthesis of Cobalt Mb

Continuing the synthesis of Cobalt Mb. Added 100 mg of Co-PPIX to 2 mL of autoclaved 100 mM Phosphate buffer, along with 10 drops of 0.1 N NaOH. This was left to cool to 4°C. Following, it was added dropwise to the apoprotein and let react overnight.