User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/08/27

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Making HAuCl4 and Lysozyme Stocks

  • Final [H+] wanted: 0.25 mM
  • Actual amount of HAuCl4: 0.04550 g
    • (slightly more water added to the volumetric flask)
  • Final [Lysozyme] wanted: 250 μM
  • Actual amount of Lysozyme:
  • 50 mL of 0.25 mM HCl was made as a control

Next, solutions were made with gold:protein ratios of 10:1 to 60:1. (See the table). The test tubes were placed in at 80°C for three hours, starting at 13:00.

Au Lysozyme H2O
2.5 mL 2.5 mL 0 mL
2.5 mL 1.25 mL 1.25 mL
2.5 mL 0.835 mL 1.635 mL
2.5 mL 0.625 mL 1.875 mL
2.5 mL 0.5 mL 2 mL
2.5 mL 0.415 mL 2.08 mL

Kinetics

0.5 mL of HAuCl4 stock and 0.166 mL of Lysozyme stock were added to three test tubes for a 30:1 gold to protein ratio. 4.33 mL of 80°C water was added. Kinetics runs were done every 30 minutes with 2.5 mL of sample in plastic cuvette from 800-360 nm. Started at 13:35.

This was rerun. See here for the results.




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