User:Patrick Hampson/Notebook/chem471/2016/10/18

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Ocean Optics BSA Ocean Optics Solution pH 8 [Au]=0.25 mM [BSA]=3.125 uM Vf=3000 uL Stock Concentrations [BSA]= 63.1 uM [Au]= 2.39 mM [NaOH]= 1 mM Volumes used for solution preparation: 148.9 uL BSA 255.6 uL Au 2565.4 uL H20 3 uL NaOH Results The graph above details the absorbance of our pH8 solution at wavelength 535.15 over time. The absorbance increases over time. It appears to have three stages. The beginning (0-4000 seconds) appears to be exponential. The middle (4000-8000 seconds ) appears to be linear. The end (8000-~11000 seconds) appears to be logrithmic. The graph above shows the maximum wavelength of our pH8 solution over time. The maximum wavelength is held constant at approximately 486nm until about 3800 seconds. The max wavelength then spikes to 550 nm. It immediately drops back down to about 536nm. From there is fluctuates between 532nm and 540nm for the remainder of the experiment. The graph above details the absorbance at maximum wavelength over time. As expected, it mimics the graph of general absorbance (first figure).