User:Patrick Hampson/Notebook/chem471/2016/10/11

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

Fluorescence 20161011

Sample Preparation

BSA Fluorescence Solution

  1. pH 7
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 160920

  1. [BSA]= 49.1 uM
  2. [Au]= 3.73 mM
  3. [HCl]= 1 mM

Volumes for making the solution:

  1. 190.94 uL BSA
  2. 147.64 uL Au
  3. 2661.4 uL H20

Data

Image:161011FluorPART1.PNG

This figure displays the wavelength of maximum emissions over time for our pH 7 Au NP.

Image:161011FluorPART2.PNG

This figure displays the integrated emission over time of our pH 7 Au NP

Image:161011FluorPART3.PNG

This figure displays the emission intensity across a spectrum of wavelengths within a time range of 0-1000 seconds for our pH 7 Au NP solution.

Image:161011FluorPART4.PNG

This figure displays the emission intensity across a spectrum of wavelengths within a time range of 1000-4600 seconds for our pH 7 Au NP solution.

Image:161011FluorPART5.PNG

This figure displays the emission intensity across a spectrum of wavelengths within a time range of 4600-10800 seconds for our pH 7 Au NP solution.


Personal tools