User:Pakpoom Subsoontorn/Notebook/Shape Determination in Bacteria/2008/10/26

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Enzymes(genes) involving in building E.coli cell wall Insert content here... List AmiA AmiC AmpC AmpH FtsA FtsBL FtsK FtsQ FtsW FtsZ MepA(mepA) MinC MinD MinE MreB MreC MreD PBP1a(ponA) PBP1B(ponB) PBP1c(pbpC) PBP2(pbpA) PBP3(pbpB or ftsI) PBP4(dacB) PBP5(dacA) PBP6(dacC) PBP6b(dacD) PBP7(pbpG) RodA ZipA Detail AmiA Class: Alanine amidases Function: degrade cell wall cross like at the septum by cleaving peptide moiety from the MurNAc so contribute to daughter cell separation(1-73, 1-82) Localization: AmiA-GFP was found to be dispersed throughout the periplasm of the cells AmiC Class: Alanine amidases Function: degrade cell wall cross like at the septum by cleaving peptide moiety from the MurNAc so contribute to daughter cell separation Localization: AmiA-GFP was found to be dispersed throughout the periplasm of the small cells but localized almost exclusively at the septal ring of the constricting cells (1-10) AmpC(1-76) Class: beta-lactamase Function: Affect cell shape (1-76) AmpH(1-76) Class: beta-lactamase Function: Affect cell shape (1-76) MepA(1-92) Class: LMW endopeptidase Function: Penicillin-insensitive endopeptidase MreB Function: possibly actin homolog. absence in spherical bacterium. require for rod shape formation in E.coli. Depletion of MreBCD can be suppressed by over expression FtsQAZ (1-98). MreC Function: function with MreB, essential for rod shape formation in E.coli.Depletion of MreBCD can be suppressed by over expression FtsQAZ(1-98). MreD Function: function with MreB, essetial for rod shape formation in E.coli.Depletion of MreBCD can be suppressed by over expression FtsQAZ(1-98). PBP1a(1-19) Function: transglycosylation, transpeptidation (1) PBP1b(1-19) Function: transglycosylation, transpeptidation (1) PBP1c(1-172) Function: transglycosylation; binds to PBPs1B, -2, -3 and MltA (1-172) PBP2 Function: elongation-specific TPase (1-183). Exhibit a preference for pentapeptide side-chain (1-148). Possibly help maintaining the diameter of the cell as PBP2 inhibitor, amdinocillin (mecillinam) induces the increment of pole diameter at newly formed cell poles (1-36) Localization: Spot-like pattern along lateral membrane, division site (GFP)(1-36) PBP3 (1-127) Function: cell-division specific TPase (1-183). N-terminal domain is required for folding and stability of penicillin binding module, functioning as an intramolecular chaperone (1-64). Exhibit a preference for tripeptide chain (1-16) Localization: division septum (IF, GFP)(1-200, 1-205). Have some interaction with cell division protein FtsZ,-A,-Q,-L,-W (1-118, 1-204) PBP4 (1-97) Class: LMW endopeptidase Function: control of cell shape, together with PBP5 (1-117) PBP5 (1-20) Class: LMW carboxypeptidase Function: control of cell shape (1-117, 1-129, 1-130). Stain with defective PBP5 has high frequency for branching (1-42) PBP6 (1-20) Class: LMW carboxypeptidase PBP6b (1-7) Class: LMW carboxypeptidase PBP7 (1-75) Class: LMW endopeptidase Function: control of cell shape, together with PBP5 (1-117) RodA (1-78) Class: translocase or flipase Function: possibly transport lipid-linked precursor of PG through cell wall outside. The mutant rodA(Ts) grows as sphere(1-78).Depletion effect can be suppressed by over expression FtsQAZ (1-194). [math]\displaystyle{ Insert formula here }[/math] Reference Scheffers, DJ and Pinho, MG. Bacterial Cell Wall Synthesis: New Insight from Localization Studies. Microbiology and Molecular biology review, Dec. 2005, p585-607