User:Odom:Schmidt

Dominic Schmidt
Doctoral Student
Darwin College, Department of Oncology, University of Cambridge
Cancer Research UK, Cambridge Research Institute
Li Ka Shing Centre
Robinson Way, Cambridge, CB2 0RE
phone: +44-1223404248
email: Dominic.Schmidt 'at' cancer 'dot' org 'dot' uk

Research Interests

I received my german diplom degree in Biochemistry at the Max Planck Institute for Molecular Genetics in the department for Vertebrate Genomics of Hans Lehrach in the laboratory of Marie-Laure Yaspo. The focus of my research was the analysis of gene regulatory networks, especially for human chromosome 21 encoded transcription factors. For this purpose I worked with ChIP-chip using high density oligonucleotide microarrays and developed ChIP-seq using next generation sequencing technology. We recently published ChIP-seq for RNA-polymerase II in human cells together with RNA-seq:

Myriad points of control influence gene expression; however, it has also been an unresolved question as to which of these mechanisms has the most influence globally. We recently showed that each layer of transcriptional regulation within the adult hepatocyte, from the binding of liver master regulators and chromatin remodelling complexes to the output of the transcriptional machinery, is directed primarily by DNA sequence. Although conservation of motifs alone cannot predict transcription factor binding, we show that within the genetic sequence there must be embedded adequate instructions to direct species-specific transcription:

My recent research uses ChIP-seq and RNA-seq to understand the rules
behind species-specific gene regulation.

1. Wilson MD, Barbosa-Morais NL, Schmidt D, Conboy CM, Vanes L, Tybulewicz VL, Fisher EM, Tavaré S, and Odom DT. Species-Specific Transcription in Mice Carrying Human Chromosome 21. Science 2008 Sep 11. doi:10.1126/science.1160930 pmid:18787134. PubMed HubMed [ref1]
2. Sultan M, Schulz MH, Richard H, Magen A, Klingenhoff A, Scherf M, Seifert M, Borodina T, Soldatov A, Parkhomchuk D, Schmidt D, O'Keeffe S, Haas S, Vingron M, Lehrach H, and Yaspo ML. A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome. Science 2008 Aug 15; 321(5891) 956-60. doi:10.1126/science.1160342 pmid:18599741. PubMed HubMed [ref2]