User:Nouf Abuladel/Notebook/CHEM 472/2016/02/02

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry


Objective

  • Setting up the camera and light inside the oven. Also, preparing a lysozyme stock solution to make the fiber solution.

Procedure

1) reading instruction to initiate the camera

2) making lysozyme stock solution and calculate the volume of gold, water and Lysozyme that should be added to the 20-mL glass tube.

3) doing some trial to figure out the best position of light, camera and fiber solution in the oven.

4) turning on the camera to record the formation of fibers overnight in the oven.


  • Below are the calculations for today's fiber synthesis. In addition to this reaction preparation
Gold Stock 
MW HAuCl4*H2O (g/mol)339.79
Mass used (g)0.05048
Moles used (mol)0.000148562
Volume stock (L)0.05
Concentration of stock (mol/L)0.002971247
Target concentration (mol/L)0.00025
Target volume of final (mL)10
Volume stock needed for final (mL)0.841397583
  
Lysozyme Stock 
MW Lysozyme (g/mol)14307
Mass used (g)0.03065
Moles used (mol)2.14231E-06
Volume stock (L)0.1
Concentration of stock (mol/L)2.14231E-05
Target concentration (mol/L)0.000004167
Target volume of final (mL)10
Volume stock needed for final (mL)1.945098499
  
Water 
Volume water needed for final (mL)7.213503918

The final setup of the camera in the oven.

Data

  • The fibers worked but the camera did not. This is because the battery was dead by the time we took the camera out of the oven. In order for the files to be saved, we needed to press the off button.

Observations

NOTHING WAS OBSERVED SINCE WE DID NOT HAVE RECORDS.


Personal tools