User:Noah Benjamin/Notebook/Experimental Biological Chem/2011/11/15

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Objective

Description

Protein Extraction

Cells were sonicated (30 sec ON/30 sec OFF 3 times) Solutions were made: 1 L 50 mM Tris + 1 M NaCl pH 9, filtered 1 L 50 mM Tris pH 9, filtered 4L 50 mM Tris pH 9 4L 50 mM Tris pH 9 cells were placed in dialysis tubes and in 50 mM Tris overnight in a -80 C.


Gold NPs: Testing Concentrations

green A: 250µL Au (4.17 mM) + 500µL BSA (30µM) + 9.25mL water green B: 500µL An (4.17 mM) + 1000µL BSA (30µM) + 8.50mL water Left in oven the entire time.

red A: 500µL Au (4.17 mM) + 500µL BSA (30µM) + 9 mL water red B: 1000µL Au (4.17mM) + 1000µL BSA (30µM) + 8mL water In 50min, Out 10min of the oven.

Both "Green" tubes produced purple solution, both "Red" tubes produced aggregate purple fibers.

Data

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Notes

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Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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