User:Noah Benjamin/Notebook/Experimental Biological Chem/2011/11/01
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Testing Gold NPs with HCl
1mL Au (2.9mM) + 1mL BSA (15µM) + 8mL Water
1mL Au (2.9mM) + 1mL BSA (15µM) + 7mL Water + 1 mL HCl (3M)
1mL Au (2.9mM) + 1mL BSA (15.5µM) + 8mL Water
1mL Au (2.9mM) + 1mL BSA (15.5µM) + 7mL Water + 1 mL HCl (3M)
Order: 1) Add water 2) Add BSA 3) Add Gold 4) Add HCl 5) Leave in oven 50 min, take out for 10 min, repeat.
Forward and reverse PCR primers were determined in accordance to the guidelines set forth by the Quick Change Manual.
Then a PCR reaction was carried out using previously prepared primers.
43 μL of ddH2O was added 1 μL on a 0.5 μ/μL of template DNA 1 μL of a 10mM dNTP solution 1.25 μL of a 100 ng/μL solution of forward and reverse primers 1 μL of enzymes were added to the solution 25 μL of wax was added slowly to the surface of the solution added to thermocycler
Tubes 1 and three had grey blobs. The tubes with HCl (2 and 4) did nothing (stayed clear).
This area is for any observations or conclusions that you would like to note.