User:Noah Benjamin/Notebook/Experimental Biological Chem/2011/11/01

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Objective

Description

Testing Gold NPs with HCl

Test Tubes:

1mL Au (2.9mM) + 1mL BSA (15µM) + 8mL Water

1mL Au (2.9mM) + 1mL BSA (15µM) + 7mL Water + 1 mL HCl (3M)

1mL Au (2.9mM) + 1mL BSA (15.5µM) + 8mL Water

1mL Au (2.9mM) + 1mL BSA (15.5µM) + 7mL Water + 1 mL HCl (3M)

Order: 1) Add water 2) Add BSA 3) Add Gold 4) Add HCl 5) Leave in oven 50 min, take out for 10 min, repeat.


PCR (from Maria's Notebook): PCR was conducted following the procedure outlined on Sept 20:

Forward and reverse PCR primers were determined in accordance to the guidelines set forth by the Quick Change Manual.

Then a PCR reaction was carried out using previously prepared primers.

   43 μL of ddH2O was added
   1 μL on a 0.5 μ/μL of template DNA
   1 μL of a 10mM dNTP solution
   1.25 μL of a 100 ng/μL solution of forward and reverse primers
   1 μL of enzymes were added to the solution
   25 μL of wax was added slowly to the surface of the solution
   added to thermocycler

Data

Tubes 1 and three had grey blobs. The tubes with HCl (2 and 4) did nothing (stayed clear).

Notes

This area is for any observations or conclusions that you would like to note.


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