User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/06/23/2010/06/23

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June 23rd, 2010

PCR with Rtth to amplify LuxAB from Vibrio fischeri's genomic DNA.

Reaction 1

1μL template DNA

6μL Buffer3.3x
3μL Mg(Ac)2
4μL DNTPs
3μL Forward
3μL Reverse

Reaction 2

10.5μL H2O
9μL Buffer3.3x
0.5μL Rtth Polymerase

I prepared four tubes in order to get a higher amount of amplified DNA. Then I ran an agarose gel and it showed that the four tubes had successful reactions.

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