User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/04/21

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Another restriction to the same plasmid BBa_B0015

I grew E.coli containing the plasmid BBa_B0015 (which contains a double transcription terminator) in 4ml of LB medium with 4μL of kanamycin 30 during 8h30 to 37°C.
I extracted the Plasmid with the Roche Kit High Pure Plasmid Isolation Kit.

I placed in the frigo the plasmids I obtained; I haven't made the restriction, yet.

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