User:Nancy T. Miles/Notebook/CHEM 572 Spr 2014/2014/02/04

Biomaterials Design Lab

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Observations from Jan. 28 Procedure Objective To obtain results (photos) from Jan. 28 procedure in which 12 test tubes were prepared for the formation of nanofibers, 3 of the test tubes containing PLA scaffolding. The Gold:Myoglobin nanofibers were formed with a 100:1 ratio Methods Images of the nanofiber formation were taken 7 More PLA scaffolds were created, but with taller dimensions than previous (instead of 12 layers, the new scaffolds will have ~50 layers). Procedure from Jan. 28 will be repeated with new scaffolds "Brute force" experiment begins Of the nine nanofiber solutions made on Jan. 28, combine with a short scaffold (these scaffolds will be 1/3 the height of the original scaffolds; hence, these will be 4 layers tall). Let these nanofiber&scaffold combination in solution dry overnight Results will be analyzed next lab period. Results Nanofibers without scaffold Nanofiber&12-layer scaffold interaction: Nanofiber and scaffold interaction under microscope Sample Preparation Au stock solution: 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM Myoglobin stock solution: 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM Volumes of Au, Myoglobin, and water for each test tube: Final [Au]=0.5mM, 5mL total volume