User:Monika Gasiorek/Notebook/CHEM-571 2014F/2015/02/04
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February 4th, 2015
Observations from overnight reactions...
All reactions involving the proteases proteinase K, trypsinItalic text, and chymotrypsin resulted in the formation of a purple colloidal solution from the cleavage of the solid phase AuNP fibers. All four pepsin reactions did not demonstrate any visible changes...yet again.
UV-Vis samples of all 16 reactions were run from 200-800 nm, with a large-volume quartz cuvette. The solutions were mixed before being added. The cuvette was washed twice with 0.5 M HCl, once with 1% SDS, and dried with Methanol.
Trypsin, Chymotrypsin, and Proteinase K all had discernible degradation of AuNP fibers at the conditions tested. The absorbance values were all relatively similar between the proteases. The maximum wavelengths were between 526 and 529 nm, which were smaller than expected. This means the colloid produced had smaller diameter particles. This will be confirmed with gel analysis.
Sample Preparation for Next Week
1. 0.00125 g of proteinase K was measured out and stored in the freezer to be used in the preparation of five proteinase K solutions next Tuesday.
Protocols for next week's Proteinase K reactions and analyses can be found in the entries for February 10th and 11th.