Objective
To repeat the experiment performed on 11/15/11.
Description
- Make two solutions of AuNP.
- Solution 1 (aqueous AuNP):
- Combine 0.516 mL of 30 μM BSA and 0.350 mL of 4.17 mM HAuCl4 in a test tube.
- Place in 80°C oven until solution turns purple (about 1.5 hours).
- Solution 2 (AuNP/BSA fibers):
- Combine 0.516 mL of 30 μM BSA and 0.700 mL of 4.17 mM HAuCl4 in a test tube.
- Place in 80°C oven for 3.5 hr.
- Remove 1 mL of solution 1, place remainder back in oven.
- Take UV vis data.
- Add 100 μL 50.1 mM tris buffer (pH 10.1).
- Repeat steps 3 and 4 two more times.
- After 1.5 hr, remove solution 1 from oven again.
- Take UV vis data.
- Add 100 μL 50.1 mM tris buffer (pH 10.1).
- Repeat steps 7 and 8 two more times.
Data
Purple fibers formed after about 1 hour. The purple solution was first removed for testing after about 1.5 hours. The solution was returned for another 1.5 hours, then tested again using UV-Vis and the Tris buffer.
Data taken after solution was first removed:
Data taken after solution was removed for the second time:
Notes
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