User:Michaela Harper/Notebook/Biological Chemistry Lab/2011/11/16

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Objective

To repeat the experiment performed on 11/15/11.

Description

  1. Make two solutions of AuNP.
    • Solution 1 (aqueous AuNP):
      1. Combine 0.516 mL of 30 μM BSA and 0.350 mL of 4.17 mM HAuCl4 in a test tube.
      2. Place in 80°C oven until solution turns purple (about 1.5 hours).
    • Solution 2 (AuNP/BSA fibers):
      1. Combine 0.516 mL of 30 μM BSA and 0.700 mL of 4.17 mM HAuCl4 in a test tube.
      2. Place in 80°C oven for 3.5 hr.
  2. Remove 1 mL of solution 1, place remainder back in oven.
  3. Take UV vis data.
  4. Add 100 μL 50.1 mM tris buffer (pH 10.1).
  5. Repeat steps 3 and 4 two more times.
  6. After 1.5 hr, remove solution 1 from oven again.
  7. Take UV vis data.
  8. Add 100 μL 50.1 mM tris buffer (pH 10.1).
  9. Repeat steps 7 and 8 two more times.

Data

Purple fibers formed after about 1 hour. The purple solution was first removed for testing after about 1.5 hours. The solution was returned for another 1.5 hours, then tested again using UV-Vis and the Tris buffer.


Data taken after solution was first removed:


Data taken after solution was removed for the second time:

Notes


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