User:Melvin Colorado Escobar/Notebook/chem 471/2015/11/10

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Objective

  • Running Bradford assay for 1 nM of protease (Thermolysine..)

Description

Bradford protocol was taken from Dr. Hartings here

This is our smallest concentration of Thermolysin. We chose longer time intervals to try to capture what is happening throughout the whole processes of the fibers breaking down. Since the concentration is smaller we also expect the reaction to take longer.

Data Analysis

Image:Raw Data AuNPs with 1 nM Thermolysin Absorbance vs. Wavelength.PNG

Image:Corrected data 1nM thermolysin absorbancne vs wavelength.PNG

Image:Absorbance vs time 1nm Thermolysin.PNG

Notes

This area is for any observations or conclusions that you would like to note.


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