Objectives
- Analyze solutions of porphine and either Al2(SO4)3, Fe2(SO4)3 FeSO4, or Na2SO4 in HPLC H2O.
- Obtain FTIR data on PVOH films.
Absorbance of Solutions of 1.89 μM Porphine and Varied Concentration of Salt in HPLC H2O
- The concentrations of salt in a total solution volume of 10 mL, with porphine concentration of 1.89 μM were as follows:
- 1 mM, 2 mM, 5 mM, and 10 mM Al2(SO4)3
- 1 mM, 2 mM, 5 mM, and 10 mM Fe2(SO4)3
- 1 mM, 2 mM, 5 mM, and 10 mM FeSO4
- 1 mM, 2 mM, 5 mM, and 10 mM Na2SO4
- There were 16 solutions in total.
Volumes and Concentrations of Porphine and Salt in Solution
- Initial Concentrations of Stock Solutions
| Porphine
| Al2(SO4)3
| Fe2(SO4)3
| FeSO4
| Na2SO4
|
| 9.445 uM | 10 mM | 10 mM | 10 mM | 10 mM
|
- Volumes of Stock Solutions Added to Make Sample Solutions
| Porphine
| Al2(SO4)3
| Fe2(SO4)3
| FeSO4
| Na2SO4
|
| 2 | 0.1 | 0.1 | 0.1 | 0.1
|
| 2 | 0.2 | 0.2 | 0.2 | 0.2
|
| 2 | 0.5 | 0.5 | 0.5 | 0.5
|
| 2 | 1 | 1 | 1 | 1
|
- Final Concentrations of Porphine and Salt in Sample Solutions
| Porphine
| Al2(SO4)3
| Fe2(SO4)3
| FeSO4
| Na2SO4
| Volume of Water Added [mL]
|
| 1.89 µM | 1 mM | 1 mM | 1 mM | 1 mM | 9.9
|
| 1.89 µM | 2 mM | 2 mM | 2 mM | 2 mM | 9.8
|
| 1.89 µM | 5 mM | 5 mM | 5 mM | 5 mM | 9.5
|
| 1.89 µM | 10 mM | 10 mM | 10 mM | 10 mM | 9
|
UV-Vis Spectra of 1.89 μM Porphine and Salt Solutions
|
