User:Mbennie/Notebook/Papers and Books/Autotransporters General

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Error fetching PMID 16369779:
  1. Error fetching PMID 16369779: [Autotransporter-Review]
  • Focuses on AIDA-I protein which is an an autotransporter naturally expressed in e coli
  • Discusses applications using AIDA-1: expressing enzymes(whole cell biocatalysis), enzyme inhibitors(library screening), and epitotes(vaccine development)
  • Interesting note: passenger proteins can dimerize on the cell surface

Error fetching PMID 16787545:
  1. Error fetching PMID 16787545: [Autodisplay-gramnegbac]
  • Autotransporter = N-terminal signal peptide + passenger domain + translocator domain
  • Study of autotransporters encourage because of their simplicity
  • Possible issues with autotransporters
    • Passenger must be in "translocation-competent state" in periplasm
    • Need specific signal sequences
    • Host can introduce genetic background
  • Other systems: Omp85, LamB, OmpA, PhoE, OmpC, TraT, FhuA, and intimin
  • Autotransporters (AT) belong to type V secretion system, specifically the type Va branch
  • Form beta-barrel to stabilize protein in the outer membrane
  • Passenger can undergo some level of folding in the periplasm
  • Most passenger domains are cleaved into media
  • "System is not amenable to the display of eukaryotic cDNA libraries."
  • 50,000 - 100,000 copies per cell

Error fetching PMID 9778731:
  1. Error fetching PMID 9778731: [Early-Autotransporter-Review]
  • "No energy coupling or accessory factors are known to be required for the translocation process"
  • Some interesting autotransporters in e coli
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