User:Matthew Whiteside/Notebook/Fumigatus Microarray/2009/03/12

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

Fumigatus Research

Metabolism Targets

Carbon metabolism

  1. possible players: glycogen phosphorylase (Afu1g12920), alcohol dehydrogenase (Afu3g01490), isoamyl alcohol oxidase (Afu3g07410) and NAD-dependent formate dehydrogenase (Afu6g04920).

GABA metabolism

  1. Afu5g00710 (GABA permease, putative)
    • upreg in our study, SreA, and StuA studies.
Nitrogen metabolism
  • AreA & B
    • main repressors in nitrogen repressing system (probably cooperate)
    • e.g. arginine use as N source requires agaA and otaA genes (arginase & ornithine aminotransferase), repressed when ammonium available.
  • PopB
    • deadenalyase (remove polyA tail = +mRNA decay)
    • AreA regulated by Gln (glutamine).
    • Many N metab genes are increased degraded in response to Gln by PopB
    • P-body (cytoplasmic structure) is site of action (mRNA degradation by signalling pathways).
TCA
  • GABA is catabolized to succinate via succinicsemialdehyde. potential bypass outside the classical tricarboxylic acid cycle

Iron Regulation

A. nidulans Hap complex
    • Heterotrimeric CCAATT-binding complex (CBC). Evo conserved complex of HapB, C & E.
    • A. nidulans has HapX. Repressed by GATA-factor SreA (iron regulation)
      • repressed during iron starvation.
    • Also CBC is modified by Thioredoxin A (trxA). HapC is oxidized by trxA. When reduced HapC locates to cytoplasm, and no transcription.
    • CBC is regulated by post-translation mech (trxA) and transcriptional mech (sreA) for tight control linked to iron.
A. fumigatus SreA
    • iron regulator.
    • Repressor of 49 genes, in response to iron.
    • GATA-factor (common to many ascomyota). Contains putative iron-sensing domain.
    • Repressed genes incl reductive iron assimilation and siderophore-mediated iron uptake.
    • 23 of 49 organized in clusters.
    • Special cases:
      1. HapX, iron regulator (see above)
      2. mito ornithine carrier (AmcA), supply ornithine.
      3. CgrA, ribosome biosynthesis, thermotolerance, virulence.
      4. Cluster1 - cell-wall degradation? Afu1g03350 1,3-glucanase.
      5. Pep1 - aspartic endopeptidase Afu5g13300, & putative peptidase transporter Afu1g12240. protein degradation. (Pep1 found in sera)
      6. carbon metabolism - glycogen phosphorylase (Afu1g12920), alcohol dehydrogenase (Afu3g01490), isoamyl alcohol oxidase (Afu3g07410) and NAD-dependent formate dehydrogenase (Afu6g04920).
    • motif ATCWGATAA
    • source: [1]
A. fumigatus LaeA
    • regulator of secondary biosynthesis clusters
    • Seems to act independently of SreA
    • Regulates SidE (some biosynthetic function?), but not other cluster genes (sidC, or D).
      • No homolog in A. nidulans
    • Gliotoxin biosynthesis
    • source: [1]
A. fumigatus StuA & BrlA
    • promotors of conidiation (some gene targets overlap)
    • also regulators of secondary biosynthesis (usu related to conidation)
      • StuA 6 biosynthetic clusters
      • BrlA 1 biosynthetic cluster
    • BrlA downregulates rioosomal protein synthesis in response to N limitation (not carbon)
      • seems to be TOR independent
    • source: [2]

References

Error fetching PMID 18721228:
Error fetching PMID 19028996:
Error fetching PMID 15101984:
Error fetching PMID 18678248:
Error fetching PMID 16205968:
Error fetching PMID 17845073:
Error fetching PMID 19132084:
Error fetching PMID 18490465:
  1. Error fetching PMID 18721228: [1]
  2. Error fetching PMID 19028996: [2]
  3. Error fetching PMID 15101984: [3]
  4. Error fetching PMID 18678248: [4]
    read this and all below

  5. Error fetching PMID 16205968: [5]
  6. Error fetching PMID 17845073: [6]
  7. Error fetching PMID 19132084: [7]
  8. Error fetching PMID 18490465: [8]
All Medline abstracts: PubMed HubMed


Personal tools