User:Matt Hartings/Notebook/Photosynthesis/2013/01/31

Protein Re-engineering

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MOF Abigail and I are trying a new MOF synthesis. 51.8 mg DO2A 61.0 mg Zn(NO3)2 6H20 3 mL of diethylformamide in a scint vial with a teflon cap sonicate to create a suspension Place in oven at 130C at 9:45am Abigail and I checked the reaction at 12pm, the solution was brown. This is much different than the first time we performed the reaction, where there was a clear solution with a percipitate. We are going to let it run until 1 and then let it cool. Transformation Make plates 2.5g LB 2.0g Agar 100mL water autoclave add 100uL of 100mg/ml ampicilin Pour plates