Extract hemoglobin from Ecoli previously used in expression.
- Thaw resuspended cells
- Sonicate at power level 12 on Misonix XL-2000. Sonicate for 30 seconds. Rest on ice for 30 seconds.
- Place in centrifuge tubes and balance weight to near 0.01g
- Centrifuge for 2 hours at 18000rpm and 4C
- Dialyze into 25mM Tris, 50mM NaCl pH 8.3 overnight in cold room.
- Add data and results here...
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