User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/06

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Objective

Today's schedule

  1. Ocean Optics
    1. Sarah, Anneliese, and Patrick
  2. Fluorescence
    1. Cassidy and Sara
  3. UV-Vis
    1. Hannah and Cossette


Make new gold and myoglobin stock solutions

Description

  1. Add experimental record here. Include what, how, and why...

Data

stock solutions

  1. Myoglobin
    1. 31.1 mg of myoglobin with water in 10.0 mL volumetric flask
    2. MW = 16951 g/mol
    3. concentration = 183 μM
  2. AuCl3
    1. 38.5 mg of AuCl3 with water in 25.0 mL volumetric flask
    2. MW = 303.3 g/mol
    3. concentration = 5.08 mM


samples

  1. BSA Ocean Optics
    1. 3 mL total
      1. 0.25 mM Au
      2. 3.125 μM BSA
      3. HCl or NaOH appropriate for your pH
      4. water
  2. Myoglobin Fluorescence
    1. 3 mL total
      1. 0.25 mM Au
      2. 12.5 μM Myoglobin
      3. HCl or NaOH appropriate for your pH
      4. water
  3. Myoglobin for UV-Vis during next session
    1. 5 mL total
      1. 0.25 mM Au
      2. 12.5 μM Myoglobin
      3. HCl or NaOH appropriate for your pH
      4. Appropriate amount of fructose
      5. water

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.

Hannah Harvey 09/06/16

Sarah Burkhard

Anneliese Faustino 160906

Patrick Hampson

Sara Mattson




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