User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/01

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Objective

Remaking AuNP lysozyme samples for Hannah and Cosette. In their synthesis from yesterday (lab notebooks here and here). The fault here was mine in that I gave Hannah my calculation spreadsheet from when I made BSA-AuNPs and did not tell Hannah that we needed a different concentration of lysozyme than BSA. For BSA the ratio of Au:BSA to make AuNPs is 80 (with an Au concentration of 0.25). For lysozyme this ratio is 20. With an Au concentration of 250 μM, that puts the lysozyme concentration at 12.5μM. Predictably, all of their samples resulted in fibers.

Description

  1. Add experimental record here. Include what, how, and why...

Data

stock solutions

  1. AuCl3 stock A (from 8/30)
    1. mass = 10.13 mg
    2. 10.0 mL volumetric flask
    3. concentration = 3.34 mM
  2. AuCl3 stock B (from 8/31)
    1. mass = 12.6 mg
    2. 10.0 mL volumetric flask
    3. concentration = 4.17 mM
  3. Fructose (from 8/30)
    1. mass = 23.48 mg
    2. 10.0 mL volumetric flask
    3. concentration = 13.0 mM
  4. Lysozyme stock 1 (from 8/31)
    1. mass = 8.88 mg
    2. 10.0 mL volumetric flask
    3. concentration = 62.1 μM
  5. Lysozyme stock 2 (made today)
    1. mass = 21.92 mg
    2. 10.0 mL volumetric flask
    3. concentration = 153 μM
0 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water
4 500 0 0 300B 0 10061 3194
5 50 0 0 300B 0 10061 3644
6 5 0 0 300B 0 10061 3689
7 0 0 0 300B 0 10061 3694
8 0 5 0 300B 0 4082 4287
9 0 50 0 300B 0 4082 4242
10 0 500 0 300B 0 4082 3792
11 0 0 5 300B 0 4082 4287
12 0 0 50 300B 0 4082 4242


0.0625 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water
4 500 0 0 300B 24 4082 3768
5 50 0 0 300B 24 4082 4218
6 5 0 0 300B 24 4082 4263
7 0 0 0 300B 24 4082 4268
8 0 5 0 300B 24 4082 4263
9 0 50 0 374A 24 4082 4144
10 0 500 0 374A 24 4082 3694
11 0 0 5 374A 24 4082 4189
12 0 0 50 374A 24 4082 4144

Notes

This area is for any observations or conclusions that you would like to note.


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