User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/12/04

Biomaterials Design Lab

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Objective Cassidy had previously incubated the Fe3O4 microgels with different metal salts. Today I am going to run ICP to determine the concentration of metal left in solution. Description Standards I am using the ICP multi-element standard solution VII from Millipore (1.09492.0100, Lot HC43301592) The rough concentration for all of the metal ions in this solution are 100 mg/L Solution 1 Concentration: 10 mg/L Volume of standard used: 5 mL Final Volume: 50 mL Solution 2 Concentration: 5 mg/L Volume of standard used: 2.5 mL Final Volume: 50 mL Solution 3 Concentration: 1 mg/L Volume of standard used: 0.5 mL Final Volume: 50 mL Solution 4 Concentration: 0.75 mg/L Volume of standard used: 0.375 mL Final Volume: 50 mL Solution 5 Concentration: 0.5 mg/L Volume of standard used: 0.25 mL Final Volume: 50 mL Solution 6 Concentration: 0.25 mg/L Volume of standard used: 0.125 mL Final Volume: 50 mL Solution 7 Concentration: 0.1 mg/L Volume of standard used: 0.05 mL Final Volume: 50 mL Note for solutions 6 and 7 I used a P200 pipet and added all of the solution in the tip. I did this based on a volume calibration that I had done before taking making solutions. Metal Concentration In Standards (mg/L) and wavelengths used to evaluate concentration Cd (214.439 nm) Solution 1: 10.0 Solution 2: 5.00 Solution 3: 1.00 Solution 4: 0.750 Solution 5: 0.500 Solution 6: 0.250 Solution 7: 0.100 Co (230.786 and 238.892 nm) Solution 1: 9.90 Solution 2: 4.95 Solution 3: 0.990 Solution 4: 0.743 Solution 5: 0.495 Solution 6: 0.248 Solution 7: 0.0990 Cr (267.716 nm) Solution 1: 9.90 Solution 2: 4.95 Solution 3: 0.990 Solution 4: 0.743 Solution 5: 0.495 Solution 6: 0.248 Solution 7: 0.0990 Cu (327.395 nm) Solution 1: 10.0 Solution 2: 5.00 Solution 3: 1.00 Solution 4: 0.750 Solution 5: 0.500 Solution 6: 0.250 Solution 7: 0.100 Fe (238.204 and 259.940 nm) Solution 1: 10.1 Solution 2: 5.05 Solution 3: 1.01 Solution 4: 0.758 Solution 5: 0.505 Solution 6: 0.253 Solution 7: 0.101 K (766.491 nm) Solution 1: 9.8 Solution 2: 4.90 Solution 3: 0.98 Solution 4: 0.735 Solution 5: 0.490 Solution 6: 0.245 Solution 7: 0.0980 Mg (279.553 nm) Solution 1: 10.0 Solution 2: 5.00 Solution 3: 1.00 Solution 4: 0.750 Solution 5: 0.500 Solution 6: 0.250 Solution 7: 0.100 Mn (257.610 nm) Solution 1: 10.0 Solution 2: 5.00 Solution 3: 1.00 Solution 4: 0.750 Solution 5: 0.500 Solution 6: 0.250 Solution 7: 0.100 Na (588.995 nm) Solution 1: 10.1 Solution 2: 5.05 Solution 3: 1.01 Solution 4: 0.758 Solution 5: 0.505 Solution 6: 0.253 Solution 7: 0.101 Ni (227.021 and 230.299 nm) Solution 1: 10.0 Solution 2: 5.00 Solution 3: 1.00 Solution 4: 0.750 Solution 5: 0.500 Solution 6: 0.250 Solution 7: 0.100 Pb (220.353 nm) Solution 1: 10.0 Solution 2: 5.00 Solution 3: 1.00 Solution 4: 0.750 Solution 5: 0.500 Solution 6: 0.250 Solution 7: 0.100 Zn (213.857 nm) Solution 1: 9.90 Solution 2: 4.95 Solution 3: 0.990 Solution 4: 0.743 Solution 5: 0.495 Solution 6: 0.248 Solution 7: 0.0990 Note: These wavelengths were chosen so that there was minimal overlap with the other wavelengths being used and that maximized the signal from the element of interest. Running Conditions Agilent Technologies 5100 ICP-OES Replicates 3 Pump Speed: 12 rpm Sample Introduction: Manual Uptake delay: 5s Read Time: 5s RF power: 1.20 kW Stabilization time: 5s Viewing mode: Axial Viewing Height: 8mm Nebulizer Flow: 0.7 L/min Plasma Flow: 12 L/min Aux flow: 1 L/min Make up flow: 0 L/min Sample Names hyd FeNP Pb acetate 3.79mg/L dry FeNP Pb acetate 3.79mg/L Pb acetate 3.79mg/L dry FeNP Cd acetate 1.12mg/L hyd FeNP Cd acetate 1.12mg/L Cd acetate 1.12mg/L hyd FeNP Mg SO4 1.2mg/L dry FeNP Mg SO4 1.2mg/L Mg SO4 1.2mg/L hyd FeNP Cr Cl3 2.66mg/L dry FeNP Cr Cl3 2.66mg/L Cr Cl3 2.66mg/L pulled too soon … re-running Cr Cl3 2.66mg/L hyd FeNP Zn SO4 2.88mg/L dry FeNP Zn SO4 2.88mg/L Zn SO4 2.88mg/L hyd FeNP Ni SO4 2.63mg/L dry FeNP Ni SO4 2.63mg/L Ni SO4 2.63mg/L hyd FeNP Co Cl2 2.38mg/L dry FeNP Co Cl2 2.38mg/L Co Cl2 2.38mg/L hyd FeNP Mn Cl2 1.98mg/L dry FeNP Mn Cl2 1.98mg/L Mn Cl2 1.98mg/L hyd FeNP Cu Cl2 1.705mg/L dry FeNP Cu Cl2 1.705mg/L Cu Cl2 1.705mg/L Calibration Curves Not all standards gave good data for the calibration curves. Some concentrations were too high. The following points were used: Cd (214.439 nm) Solution 2: 5.00 Solution 3: 1.00 Solution 4: 0.750 Solution 5: 0.500 Solution 6: 0.250 Solution 7: 0.100 Co (230.786 and 238.892 nm) Solution 2: 4.95 Solution 3: 0.990 Solution 4: 0.743 Solution 5: 0.495 Solution 6: 0.248 Solution 7: 0.0990 Cr (267.716 nm) Solution 2: 4.95 Solution 3: 0.990 Solution 4: 0.743 Solution 5: 0.495 Solution 6: 0.248 Solution 7: 0.0990 Cu (327.395 nm) Solution 1: 10.0 Solution 2: 5.00 Solution 3: 1.00 Solution 4: 0.750 Solution 5: 0.500 Solution 6: 0.250 Solution 7: 0.100 Fe (238.204 and 259.940 nm) Solution 2: 5.05 Solution 3: 1.01 Solution 4: 0.758 Solution 5: 0.505 Solution 6: 0.253 Solution 7: 0.101 K (766.491 nm) *Special Circumstances more info below Solution 1: 9.8 Solution 2: 4.90 Solution 3: 0.98 Solution 4: 0.735 Solution 5: 0.490 Solution 6: 0.245 Solution 7: 0.0980 Mg (279.553 nm) Solution 3: 1.00 Solution 4: 0.750 Solution 5: 0.500 Solution 6: 0.250 Solution 7: 0.100 Mn (257.610 nm) Solution 3: 1.00 Solution 4: 0.750 Solution 5: 0.500 Solution 6: 0.250 Solution 7: 0.100 Na (588.995 nm) *Special Circumstances more info below Solution 1: 10.1 Solution 2: 5.05 Solution 3: 1.01 Solution 4: 0.758 Solution 5: 0.505 Solution 6: 0.253 Solution 7: 0.101 Ni (227.021 and 230.299 nm) Solution 2: 5.00 Solution 3: 1.00 Solution 4: 0.750 Solution 5: 0.500 Solution 6: 0.250 Solution 7: 0.100 Pb (220.353 nm) Solution 1: 10.0 Solution 2: 5.00 Solution 3: 1.00 Solution 4: 0.750 Solution 5: 0.500 Solution 6: 0.250 Solution 7: 0.100 Zn (213.857 nm) Solution 3: 0.990 Solution 4: 0.743 Solution 5: 0.495 Solution 6: 0.248 Solution 7: 0.0990 For low values of K, the following standards were used Solution 4: 0.735 Solution 5: 0.490 Solution 6: 0.245 For these points the calibration curve is: Intensity = 81938.54*Concentration + 3311.66 For high values of K, the following standards were used Solution 2: 4.90 For these points the calibration curve is: Intensity = 107499.38*Concentration + 3336.50 All of the data for Na was at/near/or below the baseline value. All Na values are assumed to be zero. Which they should be ... there was no Na used anywhere in this study. Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.