User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/09/15

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Objective

Today we're going to make more fibers for analysis and use the rest of the semester.

  1. We'll be using the protocols we used on September 2nd. The only difference is that we'll use the stock concentrations that I made today (see below).
  2. I want to complete the analysis of the UV-Vis data. We need to compile a master sheet with everyone's absorbance vs concentration data in order to make a composite determination of the molar absorptivity of lysozyme
  3. I want a master spread sheet and data analysis of eppendorf tube masses. Each group needs to have measured 15-20 tubes.
  4. I also want everyone working on their writing assignment. I'll help you out with that during the class.

Description

Target Concentrations

Based on the total reaction volume that the class will be using and the final concentrations that will be in each reaction volume, I have determined that the following HAuCl4·H2O and Lysozyme masses are appropriate for today's stock solutions.

Stock solutions

  1. HAuCl4·H2O
    1. 2.5 mM
    2. MW = 339.79 g/mol
    3. Volume = 50 mL
    4. 42.4 mg
  2. Lysozyme
    1. 20 μM
    2. MW = 14307 g/mol
    3. Volume = 100 mL
    4. 28.6 mg

Actual Concentrations

The actual masses are different from the target masses. The concentrations shown below reflect the actual concentrations made for the stock solutions. I used a 50 mL volumetric flask to make the gold stock solution and a 100 mL volumetric flask to make the protein solution. In each case I used HPLC-grade water.

Stock solutions

  1. HAuCl4·H2O
    1. 50.38 mg
    2. MW = 339.79 g/mol
    3. Volume = 50 mL
    4. 2.96 mM
  2. Lysozyme
    1. 28.69 mg
    2. MW = 14307 g/mol
    3. Volume = 100 mL
    4. 20.1 μM

Directions for making samples

Note: The final concentration of gold should be 0.25 mM and the final concentration of lysozyme should be 5.556 μM. This gives a Au:lysozyme ratio of 45, which should yield fibers.

  1. 1 mL samples
    1. Volume of gold stock: 84.4 μL
    2. Volume of protein stock: 276 μL
    3. Volume of water: 640 μL
  2. 5 mL samples
    1. Volume of gold stock: 422 μL
    2. Volume of protein stock: 1,380 μL
    3. Volume of water: 3,198 μL

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


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