User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/09/04
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All of the reaction mixtures from Wednesday's lab produced a homogeneous solution of nanoparticles (AuNPs) instead of fibers (AuNP fibers).
The most likely reason is that the balance did not properly record the mass of the lysozyme. Today I am going to remake the lysozyme solution and synthesize several 1 mL samples of AuNP fibers.
Lysozyme stock solution
For these samples, I am using the gold stock solution from Wednesday and HPLC-grade water.
Trial solutions to determine if the stock is good
Samples for Students (1 mL in eppendorf tubes)
All of the samples (ratios: 45, 50, 55, 60, 65, 70) produced fibers with the new protein stock. I am going to go ahead and make the student samples with this stock.
Checked on Monday, September 8th 2015 Most of the samples produced AuNP fibers. Some produce purple solutions with some fibers crashing out of solution. I think that this is due to effects from the plastic. Will have to look in to this. But, going forward, we will only use the samples that clearly have only fibers in them.
This area is for any observations or conclusions that you would like to note.